Article
The reliable assurance of detecting somatic mutations in cancer-related genes by next-generation sequencing: the results of external quality assessment in China.
Oncotarget - 6 Sept 2016
Zhang Rui, Ding Jiansheng, Han Yanxi, Yi Lang, Xie Jiehong, Yang Xin, Fan Gaowei, Wang Guojing, Hao Mingju, Zhang Dong, Zhang Kuo, Lin Guigao, Li Jinming
Abstract excerpt
To evaluate the proficiencies of laboratories utilizing next-generation sequencing (NGS) to detect somatic mutations in cancer-related genes, an external quality assessment (EQA) was implemented by the National Center for Clinical Laboratories of China in 2015. We prepared a panel of samples that comprised eight samples made by mixing synthetic mutated DNA fragments with normal human genomic DNA and one reference...
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