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Article

An in Vitro Reconstitution System Defines the Defective Step in the Biogenesis of Mutated β-Actin Proteins.

ACS synthetic biology - 19 Nov 2021

Machida Kodai, Miyawaki Shoma, Kanzawa Kuru, Hakushi Taiki, Nakai Tomonori, Imataka Hiroaki

Abstract excerpt

In vitro reconstitution of whole cellular events is one of the important goals in synthetic biology. Using a cell-free protein synthesis (CFPS) system reconstituted with human translation factors and chaperones, we reproduced the biogenesis of β-actin, synthesis, folding, and polymerization in a test tube. This system enabled us to define which step of the β-actin biogenesis was defective in genetic mutations...

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Identifiers

PMID: 34752068
DOI: 10.1021/acssynbio.1c00432

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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