Article
Engineering of a chitosanase fused to a carbohydrate-binding module for continuous production of desirable chitooligosaccharides.
Carbohydrate polymers - 1 Dec 2021
Zhou Jianli, Harindintwali Jean Damascene, Yang Wenhua, Han Minghai, Deng Bin, Luan Hao, Zhang Wenshuai, Liu Xiaobo, Yu Xiaobin
Abstract excerpt
Chitooligosaccharides (CHOS) with multiple biological activities are usually produced through enzymatic hydrolysis of chitosan or chitin. However, purification and recycling of the enzyme have largely limited the advancement of CHOS bioproduction. Here, we engineered a novel enzyme by fusing the native chitosanase Csn75 with a carbohydrate-binding module (CBM) that can specifically bind to curdlan. The...
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