Generation and live imaging of tumors with specific genotypes in the living fly pupa.

Abstract excerpt

This protocol describes the step-by-step generation of tumors with specific genotypes on the dorsal thorax epithelium of the fly. This in vivo system allows the imaging of tumor cell morphology and behavior in high spatial and temporal resolution. Phenotypes such as cell invasion, cell division, and tumor size can be quantified and compared to specific controls or to the neighboring wild-type tissue. Thus, this...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Animals, Genetically Modified
• Share to Crosses, Genetic
• Share to Drosophila Proteins