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Article

Native Separation and Metallation Analysis of SOD1 Protein from the Human Central Nervous System: a Methodological Workflow.

Analytical chemistry - 17 Aug 2021

Roudeau Stéphane, Trist Benjamin G, Carmona Asuncion, Davies Katherine M, Halliday Glenda M, Rufin Yann, Claverol Stéphane, Van Malderen Stijn J M, Falkenberg Gerald, Double Kay L, Ortega Richard

Abstract excerpt

Studies of the metal content of metalloproteins in tissues from the human central nervous system (CNS) can be compromised by preparative techniques which alter levels of, or interactions between, metals and the protein of interest within a complex mixture. We developed a methodological workflow combining size exclusion chromatography, native isoelectric focusing, and either proton or synchrotron X-ray...

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PMID: 34348022
DOI: 10.1021/acs.analchem.1c01128

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