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Article

Structural insights into the allosteric site of Arabidopsis NADP-malic enzyme 2: role of the second sphere residues in the regulatory signal transmission.

Plant molecular biology - 1 Sept 2021

Gerrard Wheeler Mariel Claudia, Arias Cintia Lucía, E Mello Juliana da Fonseca Rezende, Cirauqui Diaz Nuria, Rodrigues Carlos Rangel, Drincovich María Fabiana, de Souza Alessandra Mendonça Teles, Alvarez Clarisa Ester

Abstract excerpt

KEY MESSAGE: NADP-ME2 from Arabidopsis thaliana exhibits a distinctive and complex regulation by fumarate, acting as an activator or an inhibitor according to substrate and effector concentrations. In this work, we used molecular modeling approach and site-directed mutagenesis to characterized the NADP-ME2 structural determinants necessary for allosteric regulation providing new insights for enzyme optimization....

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PMID: 34333694
DOI: 10.1007/s11103-021-01176-2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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