Article
Detection and identification of Giardia species using real-time PCR and sequencing.
Journal of microbiological methods - 1 Oct 2021
Jothikumar N, Murphy Jennifer L, Hill Vincent R
Abstract excerpt
We report a specific region of Giardia spp. 18S ribosomal RNA (18S rDNA) that serves as an ideal target for quantitative PCR (qPCR) detection and sequencing to identify Giardia species, including the clinically-relevant G. duodenalis, in clinical and environmental samples. The presence of multiple copies of the 18S rDNA gene and variations in the selected 18S genomic region enabled the development of a rapid,...
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