Article
Generation of Doubled Haploid Barley by Interspecific Pollination with Hordeum bulbosum.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021
Satpathy Pooja, de la Fuente Sara Audije, Ott Vivian, Müller Andrea, Büchner Heike, Daghma Diaa Eldin S, Kumlehn Jochen
Abstract excerpt
The generation of doubled haploid barley plants by means of the so-called "Bulbosum" method has been practiced for meanwhile five decades. It rests upon the pollination of barley by its wild relative Hordeum bulbosum. This can result in the formation of hybrid embryos whose further development is typically associated with the loss of the pollinator's chromosomes. In recent years, this principle has, however, only...
Topics
- Genotype
- Haploidy
- Hordeum
- Plant Breeding
- Pollen
- Pollination
- Tissue Culture Techniques