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Protocol of European Radish (Raphanus sativus L.) Microspore Culture for Doubled Haploid Plant Production.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021

Kozar Elena, Domblides Elena

Abstract excerpt

Here, we describe the first protocol of European radish (Raphanus sativus L. subsp. sativus convar. radicula) for obtaining doubled haploid plants through in vitro microspore culture, in which the full cycle of doubled haploid formation was successfully achieved. Using this protocol, a yield of up to eight embryoids per Petri dish can be obtained. Effectiveness of this protocol was confirmed for several genotypes...

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PMID: 34270014
DOI: 10.1007/978-1-0716-1335-1_13

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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