Article
Isolation of an embryogenic line from non-embryogenic Brassica napus cv. Westar through microspore embryogenesis.
Journal of experimental botany - 1 Jan 2008
Malik Meghna R, Wang Feng, Dirpaul Joan M, Zhou Ning, Hammerlindl Joe, Keller Wilf, Abrams Suzanne R, Ferrie Alison M R, Krochko Joan E
Abstract excerpt
Brassica napus cultivar Westar is non-embryogenic under all standard protocols for induction of microspore embryogenesis; however, the rare embryos produced in Westar microspore cultures, induced with added brassinosteroids, were found to develop into heritably stable embryogenic lines after chromosome doubling. One of the Westar-derived doubled haploid (DH) lines, DH-2, produced up to 30% the number of embryos...
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