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Article

A novel CRISPR-based malaria diagnostic capable of Plasmodium detection, species differentiation, and drug-resistance genotyping.

EBioMedicine - 1 Jun 2021

Cunningham Clark H, Hennelly Christopher M, Lin Jessica T, Ubalee Ratawan, Boyce Ross M, Mulogo Edgar M, Hathaway Nicholas, Thwai Kyaw L, Phanzu Fernandine, Kalonji Albert, Mwandagalirwa Kashamuka, Tshefu Antoinette, Juliano Jonathan J, Parr Jonathan B

Abstract excerpt

BACKGROUND: CRISPR-based diagnostics are a new class of highly sensitive and specific assays with multiple applications in infectious disease diagnosis. SHERLOCK, or Specific High-Sensitivity Enzymatic Reporter UnLOCKing, is one such CRISPR-based diagnostic that combines recombinase polymerase pre-amplification, CRISPR-RNA base-pairing, and LwCas13a activity for nucleic acid detection. METHODS: We developed...

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Identifiers

PMID: 34139428
DOI: 10.1016/j.ebiom.2021.103415
PMCID: PMC8213918

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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