Article
A TILLING by sequencing approach to identify induced mutations in sunflower genes.
Scientific reports - 10 May 2021
Fanelli Valentina, Ngo Kathie J, Thompson Veronica L, Silva Brennan R, Tsai Helen, Sabetta Wilma, Montemurro Cinzia, Comai Luca, Harmer Stacey L
Abstract excerpt
The Targeting Induced Local Lesions in Genomes (TILLING) technology is a reverse genetic strategy broadly applicable to every kind of genome and represents an attractive tool for functional genomic and agronomic applications. It consists of chemical random mutagenesis followed by high-throughput screening of point mutations in targeted genomic regions. Although multiple methods for mutation discovery in amplicons...
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