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Microbial diagnosis of endophthalmitis using nanopore amplicon sequencing

21 Apr 2021

Abstract excerpt

OBJECTIVES: We investigated whether nanopore amplicon sequencing of aqueous humor was capable of rapid pathogen identification in infectious endophthalmitis. METHODS: 5 cases of culture-positive bacterial endophthalmitis and 3 cases of fungal endophthalmitis (1 culture-positive and 2 presumed) were included. DNA was extracted from the aqueous humor and vitreous specimen, and PCR of bacterial rDNA (16S) and fungal...

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PMID: 33930723
DOI: 10.1016/j.ijmm.2021.151505

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