Transduction of Human T Cell Subsets with Lentivirus.

Abstract excerpt

Lentivirus-mediated gene transfer is an efficient method to introduce a variety of transgenes to human T cells. Here we describe a protocol to transduce human CD4+, CD8+, or CD4+ regulatory T cells. To illustrate the method, we use transduction with lentivirus encoding an HLA-A2-specific chimeric antigen receptor (CAR) and a transduction marker as an example. Methods to isolate, transduce, purify, and expand CD4+...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Cell Separation
• Share to HLA-A2 Antigen
• Share to Lentivirus