Article
ACoRE: Accurate SARS-CoV-2 genome reconstruction for the characterization of intra-host and inter-host viral diversity in clinical samples and for the evaluation of re-infections.
Genomics - 1 Jul 2021
Marcolungo Luca, Beltrami Cristina, Degli Esposti Chiara, Lopatriello Giulia, Piubelli Chiara, Mori Antonio, Pomari Elena, Deiana Michela, Scarso Salvatore, Bisoffi Zeno, Grosso Valentina, Cosentino Emanuela, Maestri Simone, Lavezzari Denise, Iadarola Barbara, Paterno Marta, Segala Elena, Giovannone Barbara, Gallinaro Martina, Rossato Marzia, Delledonne Massimo
Abstract excerpt
Sequencing the SARS-CoV-2 genome from clinical samples can be challenging, especially in specimens with low viral titer. Here we report Accurate SARS-CoV-2 genome Reconstruction (ACoRE), an amplicon-based viral genome sequencing workflow for the complete and accurate reconstruction of SARS-CoV-2 sequences from clinical samples, including suboptimal ones that would usually be excluded even if unique and...
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