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Generation of a common marmoset embryonic stem cell line CMES40-OC harboring a POU5F1 (OCT4)-2A-mCerulean3 knock-in reporter allele.

Stem cell research - 1 May 2021

Yoshimatsu Sho, Murakami Rei, Sato Tsukika, Saeki Tsubasa, Yamamoto Masafumi, Sasaki Erika, Noce Toshiaki, Okano Hideyuki

Abstract excerpt

POU class 5 homeobox 1 (POU5F1, also known as OCT4) is critical for maintenance of pluripotency, germ cell fate, reprogramming into a pluripotent state, and early embryogenesis. We generated an embryonic stem cell (ESC) line of the common marmoset (Callithrix jacchus) harboring a heterozygous knock-in allele of OCT4-P2A-mCerulean-T2A-pac. The ESC line (CMES40-OC) will be valuable for investigation of primed/naïve...

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PMID: 33799281
DOI: 10.1016/j.scr.2021.102308

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