Article
Introduction of glucan synthase into the cytosol in wheat endosperm causes massive maltose accumulation and represses starch synthesis.
The Plant journal : for cell and molecular biology - 1 Jun 2021
Schreier Tina B, Fahy Brendan, David Laure C, Siddiqui Hamad, Castells-Graells Roger, Smith Alison M
Abstract excerpt
We expressed a bacterial glucan synthase (Agrobacterium GlgA) in the cytosol of developing endosperm cells in wheat grains, to discover whether it could generate a glucan from cytosolic ADP-glucose. Transgenic lines had high glucan synthase activity during grain filling, but did not accumulate glucan. Instead, grains accumulated very high concentrations of maltose. They had large volumes during development due to...
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