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Article

Trans-spliced mRNA products produced from circRNA expression vectors

24 Mar 2021

Abstract excerpt

Circular (circ) RNA expression vectors are used as a method of identifying and characterizing RNA sequences that harbor internal ribosome entry site (IRES) activity. During the course of developing a vector series tailored for IRES discovery, we found evidence for the occurrence of trans-spliced mRNAs arising when sequences with promoter activity were embedded between the upstream CTD and downstream NTD exons of...

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PMID: 33762403
DOI: 10.1261/rna.078261.120

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