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Article

Generation of recombinant lentogenic Newcastle disease virus from cDNA

1 Nov 1999

Abstract excerpt

Recombinant lentogenic Newcastle disease virus (NDV) of the vaccine strain Clone-30 was reproducibly generated after simultaneous expression of antigenome-sense NDV RNA and NDV nucleoprotein, phosphoprotein and RNA-dependent RNA polymerase from plasmids transfected into cells stably expressing T7 RNA polymerase. For this purpose, the genome of Clone-30, comprising 15186 nt, was cloned and sequenced prior to...

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PMID: 10580061
DOI: 10.1099/0022-1317-80-11-2987

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