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Article

Using Next-Generation Sequencing for DNA Barcoding: Capturing Allelic Variation in ITS2.

G3 (Bethesda, Md.) - 5 Jan 2017

Batovska Jana, Cogan Noel O I, Lynch Stacey E, Blacket Mark J

Abstract excerpt

Internal Transcribed Spacer 2 (ITS2) is a popular DNA barcoding marker; however, in some animal species it is hypervariable and therefore difficult to sequence with traditional methods. With next-generation sequencing (NGS) it is possible to sequence all gene variants despite the presence of single nucleotide polymorphisms (SNPs), insertions/deletions (indels), homopolymeric regions, and microsatellites. Our aim...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Share to Polymorphism, Single Nucleotide

Source

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Identifiers

PMID: 27799340
DOI: 10.1534/g3.116.036145
PMCID: PMC5217108

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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