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Article

Multiplex CRISPR-Cas9 mutagenesis of the phytochrome gene family in Physcomitrium (Physcomitrella) patens.

Plant molecular biology - 1 Nov 2021

Trogu Silvia, Ermert Anna Lena, Stahl Fabian, Nogué Fabien, Gans Tanja, Hughes Jon

Abstract excerpt

KEY MESSAGE: We mutated all seven Physcomitrium (Physcomitrella) patens phytochrome genes using highly-efficient CRISPR-Cas9 procedures. We thereby identified phy5a as the phytochrome primarily responsible for inhibiting gravitropism, proving the utility of the mutant library. The CRISPR-Cas9 system is a powerful tool for genome editing. Here we report highly-efficient multiplex CRISPR-Cas9 editing of the...

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Bryopsida
CRISPR-Cas Systems
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Multigene Family

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PMID: 33346897
DOI: 10.1007/s11103-020-01103-x
PMCID: PMC8648701

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