Article
A one-step assay for sorted CD3+ cell purity and chimerism after hematopoietic stem cell transplantation.
HLA - 1 Mar 2021
Desoutter Judith, Usureau Cédric, Jacob Valentine, Lebon Delphine, Caulier Alexis, Da Costa Cécilia, Charbonnier Amandine, Joris Magalie, Marolleau Jean-Pierre, Guillaume Nicolas
Abstract excerpt
A hematopoietic chimerism assay is the laboratory test for monitoring engraftment and quantifying the proportions of donor and recipient cells after hematopoietic stem cell transplantation recipients. Flow cytometry is the reference method for determining the purity of CD3+ cells on the chimerism of selected CD3+ cells. In the present study, we developed a single-step procedure that combines the CD3+ purity assay...
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