Article
Structural insights into the mechanism of rhodopsin phosphodiesterase.
Nature communications - 5 Nov 2020
Ikuta Tatsuya, Shihoya Wataru, Sugiura Masahiro, Yoshida Kazuho, Watari Masahito, Tokano Takaya, Yamashita Keitaro, Katayama Kota, Tsunoda Satoshi P, Uchihashi Takayuki, Kandori Hideki, Nureki Osamu
Abstract excerpt
Rhodopsin phosphodiesterase (Rh-PDE) is an enzyme rhodopsin belonging to a recently discovered class of microbial rhodopsins with light-dependent enzymatic activity. Rh-PDE consists of the N-terminal rhodopsin domain and C-terminal phosphodiesterase (PDE) domain, connected by 76-residue linker, and hydrolyzes both cAMP and cGMP in a light-dependent manner. Thus, Rh-PDE has potential for the optogenetic...
Topics
- Choanoflagellata
- HEK293 Cells
- Humans
- Models, Molecular
- Mutation
- Phosphoric Diester Hydrolases
- Protein Domains
- Rhodopsin
- Rhodopsins, Microbial
- Structure-Activity Relationship