[Implantation of a mutant of Escherichia coli requiring diaminopimelic acid in the digestive tract of gnotobiotic mice].

doi:10.1016/s0769-2609(86)80007-2

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Article

[Implantation of a mutant of Escherichia coli requiring diaminopimelic acid in the digestive tract of gnotobiotic mice].

Annales de l'Institut Pasteur. Microbiologie - 1 Jan 2000

Ducluzeau R, Ladiré M, Raibaud P

Abstract excerpt

A DAP- auxotroph mutant of Escherichia coli DP50 requiring DAP and thymidine for growth was used as the receptor strain in genetic engineering. It failed to be implanted in axenic mice. However, when an inoculum containing more than 10(7) bacteria/ml was used, the DAP+ reverse mutant devoid of re...

Topics

Amino Acids, Diamino
Animals
Bacterial Adhesion
Clostridium
Diaminopimelic Acid
Digestive System
Escherichia coli
Genetic Engineering
Germ-Free Life
Mice
Mice, Inbred C3H
Mutation

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