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Article

Development, Optimization, and Validation of a Multiplex Real-Time PCR Assay on the BD MAX Platform for Routine Diagnosis of Acanthamoeba Keratitis.

The Journal of molecular diagnostics : JMD - 1 Dec 2020

Maubon Danièle, Richarme Claire, Post Lucie, Robert Marie G, Bernheim Diane, Garnaud Cécile

Abstract excerpt

The reported number of cases of Acanthamoeba amebic keratitis (AK) is continually increasing. Molecular diagnosis has become the first choice of ophthalmologists for identifying and confirming this clinically problematic diagnosis. However, in-house molecular diagnostic procedures are time-consuming and may not be compatible with the urgency of the situation. In this study, a previous in-house AK-PCR technique...

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Identifiers

PMID: 32976994
DOI: 10.1016/j.jmoldx.2020.09.001

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 3 Sept 2026.

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