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Use of 18S rRNA Gene-Based PCR Assay for Diagnosis of Acanthamoeba Keratitis in Non-Contact Lens Wearers in India

1 Jul 2003

Abstract excerpt

Identification of Acanthamoeba cysts and trophozoites in ocular tissues requires considerable expertise and is often time-consuming. An 18S rRNA gene-based PCR test, highly specific for the genus Acanthamoeba, has recently been reported in the molecular diagnosis of Acanthamoeba keratitis. This PCR assay was compared with conventional microbiological tests for the diagnosis of Acanthamoeba keratitis. In a pilot...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 12843065
DOI: 10.1128/jcm.41.7.3206-3211.2003

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