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Development and validation of a real-time PCR assay for the detection of clinical acanthamoebae

28 Jul 2017

Abstract excerpt

BACKGROUND: Suboptimal agreement between molecular assays for the detection of Acanthamoeba spp. in clinical specimens has been demonstrated, and poor assay sensitivity directly imperils the vision of those affected by amoebic keratitis (AK) through delayed diagnosis. We sought to develop and validate a single Taqman real time PCR assay targeting the Acanthamoeba 18S rRNA gene that could be used to enhance...

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Bacterial biofilms and quorum sensing
Legionella and Acanthamoeba research
Ocular Infections and Treatments

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 28754142
DOI: 10.1186/s13104-017-2666-x

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