Article
Localization and characterization of Citrus centromeres by combining half-tetrad analysis and CenH3-associated sequence profiling.
Plant cell reports - 1 Dec 2020
Xia Qiang-Ming, Miao Lu-Ke, Xie Kai-Dong, Yin Zhao-Ping, Wu Xiao-Meng, Chen Chun-Li, Grosser Jude W, Guo Wen-Wu
Abstract excerpt
KEY MESSAGE: The physical locations of citrus centromere are revealed by combining genetic and immunological assays for the first time and nine citrus centromere-specific markers for cytogenetics are mined. Centromere localization is challenging, because highly redundant repetitive sequences in centromeric regions make sequence assembly difficult. Although several citrus genomes have been released, the...
Topics
- Antibody Specificity
- Centromere
- Chromatin Immunoprecipitation Sequencing
- Citrus
- Cytogenetics
- Genes, Plant
- Genotyping Techniques
- In Situ Hybridization, Fluorescence
- Polymorphism, Single Nucleotide
- Tetraploidy
