Article
Deep Mutational Scanning of SARS-CoV-2 Receptor Binding Domain Reveals Constraints on Folding and ACE2 Binding.
Cell - 3 Sept 2020
Starr Tyler N, Greaney Allison J, Hilton Sarah K, Ellis Daniel, Crawford Katharine H D, Dingens Adam S, Navarro Mary Jane, Bowen John E, Tortorici M Alejandra, Walls Alexandra C, King Neil P, Veesler David, Bloom Jesse D
Abstract excerpt
The receptor binding domain (RBD) of the SARS-CoV-2 spike glycoprotein mediates viral attachment to ACE2 receptor and is a major determinant of host range and a dominant target of neutralizing antibodies. Here, we experimentally measure how all amino acid mutations to the RBD affect expression of folded protein and its affinity for ACE2. Most mutations are deleterious for RBD expression and ACE2 binding, and we...
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