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A novel droplet digital PCR human mtDNA assay for fecal source tracking

26 Jun 2020

Abstract excerpt

Human mitochondrial DNA provides a promising target for fecal source tracking because it is unique and intrinsic to humans. We developed a TaqMan chemistry assay, hCYTB484, targeting the cytochrome b gene of the human mitochondrial genome on a droplet digital PCR (ddPCR) platform and compared the performance of hCYTB484 with the HF183/BacR287 assay, a widely used assay targeting human-associated Bacteroides. For...

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Share to Bacterial Identification and Susceptibility Testing
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PMID: 32750535
DOI: 10.1016/j.watres.2020.116085

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