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Conjugation as a Highly Sensitive Assay to Study Group II Intron Splicing In Vivo.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021

LaRoche-Johnston Félix, Monat Caroline, Cousineau Benoit

Abstract excerpt

Group II introns are noncoding sequences that interrupt genes, and that must be removed or spliced-out at the RNA level during gene expression. Following the transcription of interrupted genes, group II introns self-splice while concurrently ligating their flanking exons to generate mature mRNAs ready for translation. Ll.LtrB, the model group II intron from the gram-positive bacterium Lactococcus lactis,...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 32712911
DOI: 10.1007/978-1-0716-0716-9_1

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