Article
RNA Imaging with RNase-Inactivated Csy4 in Plants and Filamentous Fungi.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020
Burnett David, Lichius Alexander, Tilsner Jens
Abstract excerpt
Subcellular localizations of RNAs can be imaged in vivo with genetically encoded reporters consisting of a sequence-specific RNA-binding protein (RBP) fused to a fluorescent protein. Several such reporter systems have been described based on RBPs that recognize RNA stem-loops. Here we describe RNA tagging for imaging with an inactive mutant of the bacterial endonuclease Csy4, which has a significantly higher...
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