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Cas9 Ribonucleoprotein Delivery via Microfluidic Cell‐Deformation Chip for Human T‐Cell Genome Editing and Immunotherapy

16 Jan 2017

Abstract excerpt

This study reports a microfluidic cell deformation-based method to deliver the Cas9 ribonucleoprotein (RNP) complexes to different cell types for efficient genome editing, including hard-to-transfect human primary CD4+ T cells. The RNP based CRISPR-Cas9 system has great advantage in shortening reaction time and reducing off-target problems, which holds great potential in future gene therapy applications.

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PMID: 32646183
DOI: 10.1002/adbi.201600007

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