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Article

Numerical optimization of microfluidic vortex shedding for genome editing T cells with Cas9

3 Jun 2021

Abstract excerpt

Abstract Microfluidic vortex shedding (µVS) can rapidly deliver mRNA to T cells with high yield and minimal perturbation of the cell state. The mechanistic underpinning of µVS intracellular delivery remains undefined and µVS-Cas9 genome editing requires further studies. Herein, we evaluated a series of µVS devices containing splitter plates to attenuate vortex shedding and understand the contribution of computed...

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PMID: 34083685
DOI: 10.1038/s41598-021-91307-y

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