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A serum-free protocol for the ex vivo expansion of Cytokine-Induced Killer cells using gas-permeable static culture flasks.

Cytotherapy - 1 Sept 2020

Palmerini Pierangela, Dalla Pietà Anna, Sommaggio Roberta, Ventura Annavera, Astori Giuseppe, Chieregato Katia, Tisi Maria Chiara, Visco Carlo, Perbellini Omar, Ruggeri Marco, Cappuzzello Elisa, Rosato Antonio

Abstract excerpt

Cytokine-Induced (CIK) cells represent an attractive approach for cell-based immunotherapy, as they show several advantages compared with other strategies. Here we describe an original serum-free protocol for CIK cell expansion that employs G-Rex devices and compare the resulting growth, viability, phenotypic profile and cytotoxic activity with conventional culture in tissue flasks. CIK cells were obtained from...

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Identifiers

PMID: 32631696
DOI: 10.1016/j.jcyt.2020.05.003

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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