Article
A serum-free protocol for the ex vivo expansion of Cytokine-Induced Killer cells using gas-permeable static culture flasks.
Cytotherapy - 1 Sept 2020
Palmerini Pierangela, Dalla Pietà Anna, Sommaggio Roberta, Ventura Annavera, Astori Giuseppe, Chieregato Katia, Tisi Maria Chiara, Visco Carlo, Perbellini Omar, Ruggeri Marco, Cappuzzello Elisa, Rosato Antonio
Abstract excerpt
Cytokine-Induced (CIK) cells represent an attractive approach for cell-based immunotherapy, as they show several advantages compared with other strategies. Here we describe an original serum-free protocol for CIK cell expansion that employs G-Rex devices and compare the resulting growth, viability, phenotypic profile and cytotoxic activity with conventional culture in tissue flasks. CIK cells were obtained from...
Topics
- Cell Culture Techniques
- Cell Death
- Cell Differentiation
- Cell Line, Tumor
- Cell Proliferation
- Cell Survival
- Culture Media, Serum-Free
- Cytokine-Induced Killer Cells
- Cytotoxicity, Immunologic