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Kinetics and function of two mutants of CLA hydrase from Lactobacillus plantarum p-8.

FEMS microbiology letters - 1 Jun 2020

Zhao Wei, Zhao Lili, Zhao Tongtong, Zhi Chao, Liu Meiqi, Jamil Khan Abdul, Bao Qiuhua, Zhang Heping, Wang Yuzhen, Zhang Feng, Zhao Guofen

Abstract excerpt

In this work, the gene of conjugated linoleic acid hydrase (CLA-HY) was cloned from L. plantarum p-8, and the protein of CLA-HY was expressed in Escherichia coli BL21. Gas chromatography-mass spectrometry was employed to verify that the purified CLA-HY can convert linoleic acid (LA) into 10-hydroxy-cis-12-octadecenoic acid (10-HOE) in the presence of flavin adenine dinucleotide (FAD). The optimal pH and...

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PMID: 32558892
DOI: 10.1093/femsle/fnaa087

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