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Calcium-Free and Cytochalasin B Treatment Inhibits Blastomere Fusion in 2-Cell Stage Embryos for the Generation of Floxed Mice via Sequential Electroporation.

Cells - 28 Apr 2020

Horii Takuro, Kobayashi Ryosuke, Kimura Mika, Morita Sumiyo, Hatada Izuho

Abstract excerpt

The generation of conditional knockout mice using the Cre-loxP system is advantageous for the functional analysis of genes. Flanked by two loxP sites (floxed) mice can be directly obtained from fertilized eggs by the CRISPR/Cas9 genome editing system. We previously reported that sequential knock-in (KI) of each loxP site by electroporation (EP) at the 1- and 2-cell embryonic stages increases the number of mice...

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PMID: 32354036
DOI: 10.3390/cells9051088
PMCID: PMC7290713

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