Article
Association of bovine major histocompatibility complex class I (BoLA-A) alleles with immune response to Brucella abortus strain 19 in calves.
Veterinary microbiology - 1 Mar 2020
Patra Biswanath, Panigrahi Manjit, Ahmad Sheikh Firdous, Dandapat Satyabrata, Kumar Pushpendra, Bhushan Bharat
Abstract excerpt
In the present study, we analyzed the immune response of calves to Brucella abortus strain 19 vaccine (S19) and its association with MHC class I (BoLA-A) alleles (exons 2-3 and 4-5). Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used for typing of BoLA-A exon 2-3 with DdeI and TaqI restriction enzymes; and exon 4-5 with HinfI in 45 crossbred calves. The PCR-RFLP analysis...
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