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Maximizing antibody production in a targeted integration host by optimization of subunit gene dosage and position

22 Jan 2020

Abstract excerpt

Historically, therapeutic protein production in Chinese hamster ovary (CHO) cells has been accomplished by random integration (RI) of expression plasmids into the host cell genome. More recently, the development of targeted integration (TI) host cells has allowed for recombination of plasmid DNA into a predetermined genomic locus, eliminating one contributor to clone-to-clone variability. In this study, a TI host...

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PMID: 31965756
DOI: 10.1002/btpr.2967

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