Article
Binding and functional profiling of antibody mutants guides selection of optimal candidates as antibody drug conjugates.
PloS one - 1 Jan 2019
Zwaagstra John C, Sulea Traian, Baardsnes Jason, Radinovic Stevo, Cepero-Donates Yuneivy, Robert Alma, O'Connor-McCourt Maureen D, Tikhomirov Ilia A, Jaramillo Maria Luz
Abstract excerpt
An increasingly appreciated conundrum in the discovery of antibody drug conjugates (ADCs) is that an antibody that was selected primarily for strong binding to its cancer target may not serve as an optimal ADC. In this study, we performed mechanistic cell-based experiments to determine the correlation between antibody affinity, avidity, internalization and ADC efficacy. We used structure-guided design to assemble...
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