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Article

C-Terminal Cysteine PEGylation of Adalimumab Fab with an Engineered Interchain SS Bond.

Biological & pharmaceutical bulletin - 1 Mar 2020

Nakamura Hitomi, Anraku Makoto, Oda-Ueda Naoko, Ueda Tadashi, Ohkuri Takatoshi

Abstract excerpt

Conjugation with polyethylene glycol (PEG) is performed to increase serum half-life of the Fab for clinical applications. However, current designs for recombinant Fab only allow PEGylation at the interchain SS bond (disulfide bond) at the C-terminal end of the heavy chain and light chain of the Fab, which the decrease of thermostability occurred by partial reduction of the interchain SS bond. An adalimumab Fab...

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PMID: 31866612
DOI: 10.1248/bpb.b19-00612

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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