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Reverse Complement PCR: A novel one-step PCR system for typing highly degraded DNA for human identification.

Forensic science international. Genetics - 1 Jan 2020

Kieser Rachel E, Buś Magdalena M, King Jonathan L, van der Vliet Walter, Theelen Joop, Budowle Bruce

Abstract excerpt

Reverse Complement PCR (RC-PCR) is an innovative, one-step PCR target enrichment technology adapted for the amplification of highly degraded (fragmented) DNA. It provides simultaneous amplification and tagging of a targeted sequence construct in a single, closed-tube assay. A human identification (HID) RC-PCR panel was designed targeting 27 identity single nucleotide polymorphisms (SNPs) generating targets only...

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PMID: 31786458
DOI: 10.1016/j.fsigen.2019.102201

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