Article
Alanine 310 is important for the activity of 1,4-α-glucan branching enzyme from Geobacillus thermoglucosidans STB02.
International journal of biological macromolecules - 1 Apr 2017
Liu Yiting, Li Caiming, Gu Zhengbiao, Xin Chenhao, Cheng Li, Hong Yan, Li Zhaofeng
Abstract excerpt
1,4-α-Glucan branching enzyme (GBE) catalyzes the formation of α-1,6 branch points in starch or glycogen by hydrolyzing α-1,4-glucosidic linkages and then synthesizing α-1,6-glucosidic linkages. In the GBE from Geobacillus thermoglucosidans STB02, alanine 310 (Ala310) is located in conserved region II. An analysis of the amino acid sequence shows that Ala310 is highly conserved in the prokaryotic GBE subfamily....
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