Article
Investigation of Streptococcus agalactiae using pcsB-based LAMP in milk, tilapia and vaginal swabs in Haikou, China.
Journal of applied microbiology - 1 Mar 2020
Pu W, Wang Y, Yang N, Guo G, Li H, Li Q, Ur Rehman N, Zheng L, Wang P, Han S, Zhou C C, Zheng J, Zeng J, Yuan J
Abstract excerpt
AIMS: To develop a pcsB-based Loop-mediated isothermal amplification (LAMP) method for the detection of Streptococcus agalactiae (GBS) in milk, tilapia and vaginal swabs. METHODS AND RESULTS: The sensitivity of the LAMP method using real-time turbidity monitoring was 1 pg of template within 1 h at 64°C, 100-fold higher than conventional PCR. The sensitivity of visual detection dropped an order of magnitude using...
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