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Development of a high-throughput γ-H2AX assay based on imaging flow cytometry

22 Aug 2019

Abstract excerpt

Measurement of γ-H2AX foci levels in cells provides a sensitive and reliable method for quantitation of the radiation-induced DNA damage response. The objective of the present study was to develop a rapid, high-throughput γ-H2AX assay based on imaging flow cytometry (IFC) using the ImageStream® X Mk II (ISX) platform to evaluate DNA double strand break (DSB) repair kinetics in human peripheral blood cells after...

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PMID: 31438980
DOI: 10.1186/s13014-019-1344-7

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