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Analysis of Paramyxovirus Transcription and Replication by High-Throughput Sequencing

7 Jun 2019

Abstract excerpt

High-throughput sequencing (HTS) of virus-infected cells can be used to study in great detail the patterns of virus transcription and replication. For paramyxoviruses, and by analogy for all other negative-strand RNA viruses, we show that directional sequencing must be used to distinguish between genomic RNA and mRNA/antigenomic RNA because significant amounts of genomic RNA copurify with poly(A)-selected mRNA....

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PMID: 31189700
DOI: 10.1128/jvi.00571-19

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