Article
Single-cell characterization and quantification of translation-competent viral reservoirs in treated and untreated HIV infection.
PLoS pathogens - 1 Feb 2019
Pardons Marion, Baxter Amy E, Massanella Marta, Pagliuzza Amélie, Fromentin Rémi, Dufour Caroline, Leyre Louise, Routy Jean-Pierre, Kaufmann Daniel E, Chomont Nicolas
Abstract excerpt
The phenotypic characterization of the cells in which HIV persists during antiretroviral therapy (ART) remains technically challenging. We developed a simple flow cytometry-based assay to quantify and characterize infected cells producing HIV proteins during untreated and treated HIV infection. By combining two antibodies targeting the HIV capsid in a standard intracellular staining protocol, we demonstrate that...
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