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Dynamic Methylation Changes of DNA and H3K4 by RG108 Improve Epigenetic Reprogramming of Somatic Cell Nuclear Transfer Embryos in Pigs

1 Jan 2018

Abstract excerpt

BACKGROUND/AIMS: DNA methylation and histone modifications are essential epigenetic marks that can significantly affect the mammalian somatic cell nuclear transfer (SCNT) embryo development. However, the mechanisms by which the DNA methylation affects the epigenetic reprogramming have not been fully elucidated. METHODS: In our study, we used quantitative polymerase chain reaction (qPCR), Western blotting,...

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PMID: 30355946
DOI: 10.1159/000494598

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