Article
Multiple mutations in the aglycone binding pocket to convert the substrate specificity of dalcochinase to linamarase.
Biochemical and biophysical research communications - 12 Oct 2018
Tongtubtim Nusra, Thenchartanan Pornpanna, Ratananikom Khakhanang, Choengpanya Khuanjarat, Svasti Jisnuson, Kongsaeree Prachumporn T
Abstract excerpt
Dalcochinase from Dalbergia cochinchinensis Pierre and linamarase from Manihot esculenta Crantz are β-glucosidases which share 47% sequence identity, but show distinct substrate specificities in hydrolysis and transglucosylation. Previously, three amino acid residues of dalcochinase, namely I185, N189 and V255, were identified as being important for determining substrate specificity. In this study, kinetic...
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