Article
Rapid isolation of Arabidopsis chloroplasts and their use for in vitro protein import assays.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
Aronsson Henrik, Jarvis R Paul
Abstract excerpt
In vitro chloroplast protein import assays have been performed since the late 1970s, initially with plant species (e.g., pea and spinach) that readily provide an abundant source of starting material and also, subsequently, a good yield of chloroplasts for import assays. However, the sequencing of the Arabidopsis genome paved the way for an additional model system that is more amenable to genetic analysis, as a...
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